Recently, the applications of green synthetic processes in field of biological, chemical and pharmacological sciences have achieved essential roles. One of the major applications of green chemistry is the synthesis of zero valent metal and metal oxide nanoparticles using natural and abundant biologicalmaterials. In the present study, we have carried out the successful synthesis of copper (II) oxide nanoparticles (CuONPS) by using the leaf extract of Urtica dioica and formation of nanoparticles confirmed by a visual color change of the solution and then UV-Visible spectroscopy. The synthetic method was found very low cost, effective, efficient and eco-friendly. The synthesised nanoparticles have been characterised by using different analytical methods such as FTIR, UV-Visible, powder XRD and FESEM. The antibacterial activity of CuONPSwas checked by well diffusion method against S. aureus, S. mutans and E. coli. The maximum zone of inhibition was found for S. mutans after incubation period and at specific dosage of copper nanoparticles.
Keywords: Green synthesis, CuONPS, Characterisation, Antibacterial activity.