Due to widespread medicinal importance and wide region of Morus alba and Andrographis paniculata, the present work has been undertaken to study the phytochemicals and their antimicrobial efficacy in their leaf extracts. The leaves samples of both the selected plant were subjected to qualitative and quantitative phytochemical analysis for the presence of various secondary metabolites by various spectrophotometric techniques. Phytochemical screening revealed the presence of flavonoids, tannins, phlobatannins in Andrographis paniculata and tannins, reducing sugars, alkaloids, phlobatannins and saponins in Morus alba. The present investigation is also reveals that both the selected samples exhibit potent antimicrobial activity against Escherichia coli. So it is also proved as a source of certain antimicrobial molecules.